Proteaseassay-资讯wiki版

来自 : 发布时间：2024-05-20

实验概要 In certain fruits, such as pineapples and mangoes, the flesh contains protein-digesting enzymes (proteases). These may play a part in helping to soften the fruit tissues as the fruit ripens, making it even more attractive to animals that might disperse the seeds. So perhaps the activities of these proteases enzymes will increase during the ripening process. &nb......阅读全文 夹心ELISA-1

Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies with the sensitivity of simple enzyme assays, by using antibodies or

由实验室自动化与筛选协会(SLAS)主办的\"SLAS2012亚洲会展”将于2012年6月19至21日在上海金茂君悦大酒店举行，大会将围绕实验技术创新推动药物及生命科学研究为主题展开。本届大会预计吸引约350名参会者及50家参展企业出席，致力于汇集行业从业人士，包括但 FDA和NIST对细胞治疗中的细胞计数

近年来，由于细胞治疗方法的兴起，人们日益需求能够高质量、稳健、有效进行细胞表征测定的方法。细胞计数，作为细胞疗法中最基础的检测项目，现今需要更高的检测可信度。2017年4月，美国国家标准与技术研究院（NIST） 食品与药物管理局（FDA）共同举办了一场专注于细胞计数的研讨会，聚焦于选择、设计

蛋白酶抑制剂资料 Protease Inhibitors

Protease Inhibitorsfrom Alberts Lab Protocols (Kathy Miller/Chris Field)Protease inhibitors are rapidly inactivated in aqueous solutions and shou

Chip Q-PCR定量分析方法分享

相关专题Q-PCR定量分析CHIP最近做了CHIP，用Q-PCR方法进行定量分析，现将我对一些文献资料的总结传上，希望对大家有帮助！i. Normalizeeach ChIPDNAfractions’ Ct value to the Input DNA fraction Ct

Eccles:Protein Lysates from Cells in Culture

Cell Lysis Buffer5mL 0.1M Tris HCl pH 8 (10mM)0.44g NaCl (150mM)0.02g EDTA (1mM)0.5mL nonidet P40 (1% w/v)0.05g SDS (0.1% w/v)Make up to 50mL with MQH

Alexa Fluor® 488 Annexin V/Dead Cell Apoptosis Kit

实验概要Apoptosis is a carefully regulated process of cell death that occurs as a normal part of development. Inappropriately regulated apopto

酶联免疫检测技术的应用现状和发展前景

酶联免疫检测技术的应用真菌毒素的检测：黄曲霉毒素 B 1 、 M 1 以及 T-2 毒素，脱氧雪腐镰刀菌烯醇（呕吐毒素 DON ），二乙酰草镰刀菌烯醇（ DAS ），玉米赤霉烯酮，赫曲霉毒素 A （ OA ）。农药的检测：主要有除草剂与杀虫剂两大类，例如杀暝松（ FN ）、甲

酶标仪在植物领域的三种应用总结

一、简介 在基于哺乳动物细胞的研究中，酶标仪主要应用于: (1) 常规分子检测，如核酸、蛋白浓度及酶活性分析等；(2) 信号转导研究， 如一些细胞信号事件如 ROS，修饰的检测；(3) 整体细胞水平的分析，如细胞的活力、凋亡和杀伤等。然而在植物领域中，酶标 仪的应用则偏向前两个方向，此外

SREBP control of lipid synthesis

Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol

llumina & 赛默飞：两大巨头NGS检测同日获临床批准 赛默飞世尔Oncomine Solid Tumor Fusion Transcript kit利用Ion AmpliSeq技术，检测ALK、ROS-1、RET和NTRK1基因的重排。OncoNetwork联盟和赛默飞世尔的研究人员开发了这一试剂盒，并对肺癌进行了验证。它适用于FFPE组 Adventitious Root Induction in Arabidopsis thaliana as a Model for In...

Adventitious root formation, the development of roots on non-root tissue (e.g. leaves, hypocotyls and stems) is a critical step during micropropag

Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates

AbstractThe kinetic characteristics and regulation of aspartate carbamoyltransferase activity were studied in lysates and cell extracts ofHelico

Comprehensive identification of novel proteins and N-glycosylation sites五

RJ provides efficient energetic fuels for the fast development of larvae and the egg-laying queen through the metabolism of sugars, lipids, and pr

Comparison of Enzymatic and Non-Enzymatic Means-2

MTT Assay on Reattached CellsMSC were seeded in 12-well cell culture dishes with 5.0 × 104cells per well (≈4.8cm2). After 5 to 6days

人白细胞介素6(IL-6)酶联免疫分析（ELISA）试剂盒使用说明

目的：本试剂盒用于测定人血清，血浆及相关液体样本中白细胞介素6（IL-6）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中人白细胞介素-6（IL-6）水平。用纯化的人白细胞介素-6（IL-6）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入白细胞介素-6（IL-6），再与HRP 标记的白

Methods for the Detection of D-Amino-Acid Oxidase-1

AbstractFour methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are de

实验室自动化与筛选协会2013亚洲会展新品发布 2 kinds of newly launched products are available to SLAS Exhibitors to distribute on 2013 SLAS Asia Conference Exhibition website. This 赛默飞牵手诺华、辉瑞开发基于NGS的伴随诊断新市场

赛默飞11月18日宣布与诺华、辉瑞两大制药公司签订长期合作协议共同开发和商业化基于基因测序的伴随诊断市场，此次协作将重点针对多个非小细胞肺癌药物项目的开发。 在此次协议中，赛默飞将利用Ion PGM Dx系统（被FDA列为二类医疗器械）和Oncomine assays开发伴随诊断市场。诺华肿瘤

植物（Plant）生长激素（GH）ELISA检测试剂盒使用说明

本试剂盒只能用于科学研究，不得用于医学诊断植物（Plant）生长激素（GH）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被生长激素（GH）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，T

Methods for the Measurement of a Bacterial Enzyme Activity in Cell Lysates3

Different enzyme assays for ACTase study in H. pyloriACTase properties were studiedin situin cell-free extracts to obtain information on e

荧光测DNA浓度

Steve HahnLast updated9/30/98This Dye measures DNA concentration using a fluorescent dye which binds only double stranded DNA. The fluorescence

Angiotensin Protein Kinase Assay

James Hardwick s angiotensin assay protocolThis specific procedure was developed to assay the activity of the Lck kinase expressed from a retrovir

DNA Fragmentation Assays for Apoptosis

Protocol I:Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 target cells (40 wells of 105 per well) with desired concentration o

Comparison of Enzymatic and Non-Enzymatic Means-1

Comparison of Enzymatic and Non-Enzymatic Means of Dissociating Adherent Monolayers of Mesenchymal Stem CellsThe dissociation of adherent mesenchymal

Fluidigm推出自动化文库制备体系 可同时检测53个基因突变

首个商业化的靶向NGS文库制备方法上市啦。利用微流控技术，通过高效的NGS文库制备工作流程，可同时检测体细胞中53个实体瘤相关基因的突变。 Fluidigm公司近日推出了AdvantaTM Solid Tumor NGS Library Prep Assay，该方法根据目前肿瘤治疗方法和已发表

荧光素酶检测（Luciferase assay）

Introduction Luciferase can be used as a reporter gene to measure the activity of promoters, and/or the transfection efficiency. Aims You will be prov

cell proliferation assay

cell proliferation assaybefore start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer s medium MM (maintenance medium) until c

植物（Plant）β-1,3-葡聚糖酶（β-1,3-glucanase）ELISA检测试...

植物（Plant）β-1,3-葡聚糖酶（β-1,3-glucanase）ELISA检测试剂盒使用说明本试剂盒只能用于科学研究，不得用于医学诊断植物（Plant）β-1,3-葡聚糖酶（β-1,3-glucanase）ELISA检测试剂盒使用说明书检测原理试剂盒采用双抗体一步夹心法酶联免疫吸附试验（EL

ELISPOT Protocol

实验概要The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune